ࡱ> a kbjbj .M\M\_b% VVVVVjjj8.jqXt"$$$\'!WWWWWWW$Y\W-V!!!WVV$$+XG'G'G'!V$V$WG'!WG'G'NFRV$6=">S0WAX0qXT?]$?]`VVF?]V@W!!G'!!!!!WWG'!!!qX!!!!?]!!!!!!!!! > : JACKSON STATE UNIVERSITY INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC) APPLICATION FOR USE OF VERTEBRATE ANIMALS IACUC USE ONLY: Protocol No.: _______________________ Approval Date: ____________________ Department: _______________________ Expiration Date: ____________________ 1. Protocol Title: Anticipated start date of study: ________ Anticipated end date of study: ______________ Study duration: ( < 1 year ( 1 year (1-2 years 2-3 years Protocol Status: qNew ____Revision ____ 2. Principal Investigator: ___________ ________________ School/Dept.: ________ _________ Contact. No.: _______________________E-mail Address: _________________ 3. Co-Investigator(s): .: __________ _____________________ 4. External Funding Source: ______________ __________ Awarded ____Pending ____Other____ 5. Will any outside contracts be used in this study? No____ Yes ____ If YES specify the outside contracts to be used: __________________________________________________________ 6. Is any research related to this study being conducted at another institution or facility? NO___ Yes___ If YES, list other involved institution(s), investigator(s) and their respective phone number (s). 7. Has this protocol undergone peer review? Has it been evaluated for scientific merit and experimental design? NO____ Yes ____If YES, list the individuals who provided the peer review. Name and Title: _________________________; Institution: ____________________; Date ______________ 8. If supported by Departmental funds or industrial sources that do not supply external peer review or if the procedures proposed have not received a review for scientific merit, two reviewers not associated with the project must perform such a review for scientific merit. If there is no conflict of interest, one reviewer should be the department chairman/section head. The undersigned have reviewed this proposal and find it to be of scientific merit. Reviewer ___________________________ Date ___________________ Reviewer ___________________________ Date ___________________ 9. Training List all individuals involved in the study who will perform procedures in live animals, or are responsible for their welfare. State their duties. The IACUC must determine if individuals are properly trained to appropriately discharge their duties. NOTE: The IACUC sponsors ongoing training seminars. Details regarding the seminars can be obtained by contacting the IACUC office. NameRole and DutiesDescription of Training 10.Occupational Health List all individuals who will be involved in the proposed study who will be exposed to animals, animal wastes, body parts, or body fluids, and/or personnel exposed (in same room/area) to aerosolized particles. These individuals must be enrolled in the Jackson University Occupational Health Program for Personnel Caring For or Using Laboratory Animals. It is the responsibility of the Principal Investigator to enroll these individuals and to ensure that the IACUC is notified of the addition of any new employees with animal exposure. NameAre you currently in the JSU Occupational Health Program? Yes or No Emergency contact: List the person(s) to be contacted in case of an emergency or clinical deterioration outside of normal working hours (list at least two persons). Name/phone number(s): ____________________________________________________. 11. Objectives/Hypothesis: In language that will be understood by members of the general public, state the scientific aims of the study and/or hypothesis to be tested. 12. Experimental Design and Methods: In language that will be understood by members of the general public, provide a succinct outline of the formal scientific plan and direction for experimentation. If several experimental groups or sequential studies are to be included in the protocol, a description of the experimental design for each separate experimental group should be contained in sub-parts to this section. For each experimental group, justify the number of animals required for this research project. Be specific. When an inferential statistics is used, show the result of the power calculation including values for means (or difference between means) and variance for parametric statistics used to determine the proposed number of animals. For other experiments, supply appropriate justification. Diagrams or figures may be helpful in communicating your intentions. The specialized language of grant proposals is generally not acceptable. 13. Number of animals requested and categories of manipulation: Provide the total number animals anticipated to be used for the approval term of this protocol. (3 years). Individual animals are categorized by the most severe manipulation category the animal will experience, i.e. an animal that will have injection B and a non-survival surgery C would be listed under C. See A-E below: 105 [A] Live animals will: receive non-painful manipulations, be held for observation as needed in herds for agricultural research; experience no treatments, manipulations, etc., be humanely killed to obtain tissues, cells, etc., receive special diets which do not result in appreciable clinical signs of pain or distress. Dead animals or parts of animals will be obtained for study. [B] Live animals will receive painful or stressful stimulus of SHORT duration without anesthesia, which results in a short-term response. Examples include but are not limited to: injections, field trapping/tagging, polyclonal antibody production, behavioral studies, short-term pain/stimulus tests as used in analgesic evaluation, tattooing, blood sampling, tail-tipping for genotyping, and standard agricultural husbandry practices (i.e. dehorning, branding, castration). [C] Live animals will have significant manipulations, surgery, etc., performed while anesthetized. The animals will be killed humanely at the termination of the procedure without regaining consciousness. [D] Live animals will have manipulations performed while anesthetized and are allowed to recover and/or animals will develop discernable clinical signs indicating pain, distress, or significant physiological changes spontaneously or as a result of specific experimental procedures. Examples include, but are not limited to: Survival surgical procedures of any type, studies which would include tumor development (including Ascites), dietary studies resulting in discernable clinical signs of pain or distress, mother/infant separation, prolonged restraint of conscious animals for purposes other than routine clinical procedures, studies utilizing hazardous materials in live animals (Carcinogenic Agents, Radioactive Agents, Infectious Agents, Toxic Chemicals, etc.), restrictions of food/water intake for more than 12 hours, severe climatic stress, drug addiction, etc. PALLIATIVE TREATMENT MUST BE DESCRIBED AND WILL BE PROVIDED AS NEEDED OR REQUIRED BY THE IACUC. [E] Live animals will experience significant/severe pain or distress. Examples as in [D] above, or the use of paralytic drugs, WITHOUT BENEFIT OF ANESTHETICS, TRANQUILIZERS, OR ANALGESICS. Significant scientific justification is required for using this Manipulation Category and criteria for moribundity and euthanasia must be provided. Experimental Group SpeciesCategory of ManipulationNumberMaximum Days per Animal Justification for Category of Manipulation: Describe how the category of manipulation for each experimental group of animals was determined. Each experimental group should be categorized according to the most severe procedure that group of animals will experience. 14. Will any of the experiments described in this protocol involve the euthanasia/experimental death of pre-weanling animals (animals born/hatched, but euthanized prior to separation from parent)? No___; Yes ___ The United States Department of Agriculture (USDA) and the American Association for the Accreditation of Laboratory Animal Care (AAALAC) require annual reporting of the numbers of each species of animals used in research and teaching. 15. RECORDS & MONITORING: State where the aforementioned records will be available for inspection by the IACUC, Attending Veterinarian, and federal officials. For daily animal monitoring, describe your record keeping system for observation of animal health and welfare. 16. HOUSING: List the location of the general housing facility: ____________________________________ . Will the animals be in a lab outside of the general housing facility listed above at any time? Yes. If yes, where will the animals be? For how long and why the specified length of time? Will the alternate housing meet IACUC standards for animal care and welfare? Also, describe how the animals will be transported to/from the lab. 17. METHODOLOGY: Please check all the procedures that will be implemented and provide additional information as requested.  FORMCHECKBOX  Field Observation Only:  FORMCHECKBOX  Tissue, Organ, Device, Etc. Collection: Describe materials being collected: Items to include:  FORMCHECKBOX  Breeding: Rodent Breeding Program section of this form must be completed.  FORMCHECKBOX  Live Capture of Wild Animals: Complete section on restraint of movement. Describe method of capture: . Will animals be tagged? Method: .  FORMCHECKBOX  Fasting and/or Water Deprivation: Why will animals be deprived of food and/or water? What will be the duration/frequency of deprivation and describe the possible effects of deprivation.  FORMCHECKBOX  Special Diets: Detail the composition of the special diet and any special equipment needed: __________________ Who will prepare the diets? How often are animals fed? _____ Briefly describe the rationale for utilizing a special diet and possible affects on the animals health .  FORMCHECKBOX  Restraint of freedom of movement while awake: Why will activity be restricted? Method of restriction: Duration: Frequency: Frequency of monitoring during restriction:  FORMCHECKBOX  Bleeding Frequency of bleeding: . Volume of blood drawn: Method of collection: Total blood draws per animal: Will Fluids be replaced? .  FORMCHECKBOX  Tail-tipping Initial the Regimen you will be following: Anesthesia will not be required for tail tipping under the following conditions: Less than 1cm of the intact tail has been or is being removed. It is recommended that each tail tipping should remove .5cm or less at a time. No more than 2 tail tippings per animals. If more than 2 tail-tippings need to be performed on an individual mouse, complete the information below: If more than 1 cm of the tail is being removed, complete the information below: In the event that more than 2 tail-tippings need to be performed on an individual mouse and/or more than 1 cm of the tail is being removed, the investigator must provide scientific justification for utilizing these methods. Use anesthesia for the procedure: (Regimens appropriate for this procedure include administration of a topical anesthetic to the tail tip or administration of an inhalation anesthetic. Agent: Dose: . Duration of Action: .  FORMCHECKBOX  Immunization/Antibody/Ascites Production and Collection Monoclonal/Polyclonal antibody production: Agent(s) used: . Volume injected: _______ Site of injection: Frequency of injection:______ Frequency of fluid withdrawal: Volume of fluid withdrawn: _______ Maximum number of collections: Frequency of observation: ________ Adjuvant(s) used: .  FORMCHECKBOX  Inoculation of biological materials of mammalian-origin into rodents: Which of the following biological materials will be inoculated:  FORMCHECKBOX  tumors  FORMCHECKBOX  cell lines  FORMCHECKBOX  hybridomas  FORMCHECKBOX  embryonic stem cells  FORMCHECKBOX  blood or serum  FORMCHECKBOX  monoclonal or polyclonal antibodies  FORMCHECKBOX  other . What is the host species origin of these biological materials?  FORMCHECKBOX  mouse  FORMCHECKBOX  rat  FORMCHECKBOX  hamster  FORMCHECKBOX  rabbit  FORMCHECKBOX  human  FORMCHECKBOX  other . What species will be inoculated with the biological materials?  FORMCHECKBOX  mouse  FORMCHECKBOX  rat  FORMCHECKBOX  hamster  FORMCHECKBOX  other . Have these biomaterials been passed through rodents previously?  FORMCHECKBOX  Yes  FORMCHECKBOX  No If yes, approximately how many times have they been passed? .  FORMCHECKBOX  Test Drug administration Drugs to be administered: Amount and route of administration: .  FORMCHECKBOX  Palliative Therapy for Non-Surgical Protocols (Complete for all studies in section 13 of categories of animal manipulation) Federal laws require that animals which will experience more than momentary pain and distress (such as that associated with a needle-prick) must be provided with appropriate sedatives, analgesics, and/or anesthetics. Withholding of these agents required scientific evidence that such drugs will interfere with the study or harm the welfare of the animal. Such justification must be provided in writing by the Principal Investigator. Any change from the methods or agents listed herein must be pre-approved by the animal care veterinarian and such approval documented in the animals record. Permanent changes in palliative therapy must be submitted to the IACUC as an amendment to the protocol. Clinical signs of pain or distress that will be used to evaluate the need for palliative therapy should be listed under Section 18. SURGICAL PROCEDURES section: Surgical Procedures, Anesthetic Regimen, Peri-operative analgesics, Post-Operative Care  FORMCHECKBOX  Surgical Procedures:  FORMCHECKBOX  Survival?  FORMCHECKBOX  Non-Survival?  FORMCHECKBOX  Multiple Survival Surgery? (see below) Site of surgical facilities that will be used in this study: ________________  FORMCHECKBOX  Investigator Lab (bldg/room #) _______ , Other: Please provide the names and qualifications of individuals participating in the surgical procedures:  FORMCHECKBOX  NPO: When should food and/or water be removed?  FORMCHECKBOX  Anesthetic Regimen Drug: N/A Dose: . Route of Administration: Duration: . Monitoring Processes: . Tranquilizers: Dose: . Route of Administration: Duration of Effect: . Frequency of Administration: Number of days administered: Describe in detail the surgical procedures. Will any individual animal be subjected to more than one major survival surgical procedure?  FORMCHECKBOX  Yes  FORMCHECKBOX  No Briefly describe what will occur during each surgical event, stating the interval between the surgical events. **Provide Scientific Justification for having multiple survival surgical events. Pre-Surgical Analgesia Administration. Agent: N/A Dose: Route of Administration: . If pre-surgical analgesia will not be administered, scientific justification must be provided below: Post-Operative Care Will intensive care be required?  FORMCHECKBOX  Yes  FORMCHECKBOX  No If yes, for how long? . Who will provide Post-Operative Care? Analgesics: Dose: . Route of Administration: Duration of Effect: . Frequency of Administration: Number of days administered: . If post-operative analgesia will not be provided, scientific justification must be provided below: Antibiotics: Dose: . Route of Administration: Duration of Effect: . Frequency of Administration: Number of days administered: . Briefly describe post-operative monitoring procedures - Include names of participating personnel Provide Scientific Justification for not providing pre- or post-operative analgesia. PI signature:_____________________ 19. Pain A. Would a non-anesthetized animal feel more than momentary or slight pain or distress during any procedure in the protocol? No_____; Yes _____ If yes, continue below; if No, proceed to question #19. B. Federal regulations require that the principal investigator consider alternatives to procedures that may cause more than momentary or slight pain or distress to the animals. Please provide a written narrative of the methods and sources used to determine that alternatives were not available. Sources might include Biological Abstracts, Index Medicus, the Current Research Information Service, or the Animal Welfare Information Center of the National Agriculture Library (301/504-6212). C. Will appropriate analgesia or anesthesia be used? Yes____; No ____If no, provide justification. 20. Prolonged Restrain: Will prolonged restraint of unanesthetized animals be used? Yes_____ No _____ If yes, provide justification. 21. Euthanasia: Describe the method used. If appropriate, give generic rather than brand names of agents used, dosage (mg/kg) and routes of administration. If euthanasia is not a scheduled part of this protocol, please describe the method to be used in the event it becomes necessary. The method of euthanasia used should be consistent with the recommendations of the 2000 Report of the AVMA Panel on Euthanasia (JAVMA 218:669, 2001: Appendix III in the Animal Resources Program Information Manual). If method is not recommended by AVMA, provide detailed scientific justification. 22. Hazardous Agents A. Will hazardous agents be used in live animals? Yes____ No _____If yes, answer the following: 1. Has the approval been obtained from the Biosafety Committee? Yes_____ No _____ Date of approval: _____________ 2. Name of agent: Arsenic trioxide 3. Carcinogens(s): __________________________________ 4. Infectious agent(s): _______________________________ 5. Toxic chemical(s): ________________________________ 6. Transplanted tissues, cells or fluids: State human or animal: _________________________ If human, will IRB (i.e. Clinical Research Practices Committee) approval be needed for the project? Yes____ No _____. Date of approval? ___________ In this research, hazardous arsenic trioxide will be involved. These compounds will be obtained from Chem Service, USA. Because of their carcinogenic toxic potential, special care will be taken to follow the NIH Guidelines for Laboratory Use of Carcinogenic Chemicals. The chemicals will be purchased, received and stored in a designated cabinet in the laboratory. Upon receipt, information on the receipt date, amount, and physical state will be recorded in a lab notebook. Wastes containing arsenic trioxide treated mice will be considered hazardous wastes, and will be stored in a special container for an appropriate disposal. 23. Radioisotopes: Will radioisotopes be used in live animals? Yes____ No____ . If yes, you must complete the form Radionuclide Use in Animals: and obtain Radiation Safety Committee approval before receiving final approval of this ACUC application. Signature Section I hereby apply for a three-year approval (with annual renewal required for the second and third years) for the project described and assume responsibility for the animal care and use associated with this research. I certify that the activities listed in this protocol do not unnecessarily duplicate previous experiments. I certify that all personnel involved in the animal care, treatment and use aspects of this protocol are, or will be, adequately trained prior to participation in this study. These animal experiments conform to all policies of Ƶ. I certify that all employees who will be in direct contact with, or exposed to animals, their wastes, body parts, or body fluids, and/or exposed (in the same room/area) to aerosolized particles from animals will be enrolled in the JSU Occupational Health Program, and I will ensure that the Animal Resources Director is notified of the addition of any new employees. In addition, if my proposal is funded, I will complete forms required by the veterinarian for facilitating the welfare of animals within my study. And, I will comply with the policy for acquiring animals for my study. _______________________________ ________________ Principal Investigator Date _____________________________________________________ ________________ Responsible Faculty Advisor if P.I. is not faculty. Date     PAGE  PAGE 6 Mw) = > ] ^ ` h j k o Ǹ֣֜{ooao֣U֣h(@4h~CJ\aJhxh~5CJ\aJ jh~CJ\aJhmh~5CJ\aJhmh5E5CJ\ hjCJ\ h5ECJ\h5ECJ\aJhj5>*CJ\aJhmh5E5>*CJ\aJhmh~5>*CJ\aJh~CJ\aJh~>*CJ\ h~CJ\h~5CJaJ h~5CJMwx' ( ) = > {q  ^` `#$d%d&d'dNOPQ' $d%d&d'dNOPQ` *$ $d%d&d'dNOPQ` a$$a$    $ , . 2 f h r ~    < Z \ h ~ öÉtgg`YMh[Sh5E>*CJ\ hjCJ\ hR,oCJ\h[Sh5E5>*CJ\hmhj5CJ\hj5CJ\hmh5E5CJ\hoh~CJ\hmhj5>*CJ\hj5>*CJ\hmh5E5>*CJ\ h~CJ\ h~5CJh~>*CJ\ hW>CJ\hW>CJZ\aJh~CJ\aJh~CJZ\aJ 0 2 T WXde ^ gdW> ^  ^`gdW> ^`gd~ ^`gd5E  ^` ~    % & 0 1 2 : > E J O S T e i j T¶xoxfxoxhW>CJ\aJh~5CJaJh~CJ\aJ hI5CJhICJZ\aJh,hI5>*CJ\h,hj5>*CJ\hj5CJ\h,hI5CJ\ hICJ\h=Mh5E5>*CJ\h=Mhj5>*CJ\ hjCJ\ h5ECJ\ h~5CJ h~CJ\%TX9>Xd`%:VXλβΟΟΐλ·ΩssshUh~B*CJ\ph h~5h~>*CJ\hr~h~B* CJ\phhoh~CJ\h~CJ\aJh~6CJ\ h~5CJhW>h~5CJ\ h~CJ\h~CJaJh~5CJaJhW>CJ\aJh~CJ\aJ hW>CJ\,`WWW $Ifgd~kd$$IflCFh &$ \  t06    44 la $$Ifa$ ^ qhhh $Ifgd~kd$$IflFh &$ \  t06    44 laqhhh $Ifgd~kd.$$IflFh &$ \  t06    44 la !"#$;<UVXqoooooooiic$If ^ kd$$IflFh &$ \  t06    44 la X]off $Ifgd~zkdX$$Ifl0h&, t0644 la $$Ifa$$If$If{{ $Ifgd~zkd$$Ifl0h&, t0644 la{{ $Ifgd~zkdf$$Ifl0h&, t0644 laJKzzzumhgd~$a$gd~gd~$a$gd~zkd$$Ifl0h&, t0644 la QRghJKp KWBP!!!!##F%%%%C&H&I&ƿ|pf\hoh~CJ\hJXh~CJ\hJXh~CJ\aJh5th~CJ\aJhPW.h~5CJ\aJhph~5OJQJh~>*CJ\aJh~5CJaJh~CJ\aJ h~CJ\ h~5CJhF>0J5CJaJh~0J5CJaJ hF>CJ\hF>hF>CJ\ hF>CJ h~CJ$ $$%%%%&&0& $Ifgd~ 0^`00&1& kdt$$If>rx4`'(p  t20'44 lap2ytJ1&2&3&4&5&6&7&8&Bkd$$If>rx4`'(p t0'44 la $Ifgd~8&9&:&;&<&=&>&?&Bkd$$If>rx4`'(p t0'44 la $Ifgd~?&@&A&B&C&D&E&F&Bkd$$If>rx4`'(p t0'44 la $Ifgd~F&G&H&I&J&S'T'B:88$a$gd~kd5$$Iflrx4`'(p t0'44 la $Ifgd~I&J&S'((()))/)|)'*3*5*i*k**++T,U,f,,,,,,Ӹ{t{kdVE jh~CJU\aJjh~CJU\aJ h~5\h~CJ\aJ hFCJ\h~>*CJ\aJhJXh~CJ\aJhJXh~CJ\aJhJXh~5CJaJhJXh~CJOJQJ\h~CJOJQJ\h~5B*CJOJQJphfh~5CJOJQJ hJ5CJ h~5CJ h~CJ\h~CJ\aJh~T'(()())&*'*+T,U,,,,,r-s-t---4.. h^h`gd~ ^`gd~ h^h` h^h`gd~@^@h^h h^h`,,,-----7-9-:-o-p-s-t-u---------Q...////////l0۵ҙҵwlll[ j h~CJU\aJh~>*䴳a j( h~CJU\aJ jh~CJU\aJhO/hO/5CJ\aJhJXh~5CJ\aJhJXh~CJ\aJ j<hO/CJU\aJhO/CJ\aJjhO/CJU\aJh~CJ\aJjh~CJU\aJ#.//00112232s2%3344445*6666&7:88 9 h^h h^h`h^h h^h` h^h`l0000000L1|12223242B2C2D2r2s22%3<333J4U44444444415K55555)6D6666666'7/77788:8E888e9k9: ; jv h~CJU\aJ j h~CJU\aJ j h~CJU\aJ j h~CJU\aJjh~CJU\aJh~CJ\aJh~>*䴳a< 9 9::d;;;<<;<<D>>????(@)@@ p@ P  ^ `h^h h^h` 0^`0^ ;;c;x;;;;;;;I<<<<=K=j==== >D>c>>>>>??????*@+@9@:@;@C@D@R@S@T@`@a@o@p@q@}@~@@ j h~CJU\aJ jN h~CJU\aJ j h~CJU\aJ jb h~CJU\aJ j h~CJU\aJjh~CJU\aJh~>*䴳ah~CJ\aJ2@@@@@@@@@@@@@@@ A AAAAAAAAAAAAAAAA B B BBB#Bضإؚ؉xg jh~CJU\aJ jh~CJU\aJ jh~CJU\aJh~>*䴳a jh~CJU\aJ j&h~CJU\aJ jh~CJU\aJh~CJ\aJjh~CJU\aJ j:h~CJU\aJ%@AABBCCDDD E E EEEYHZHHHWI^ h^h`gd~ p@ @ ^@ ` p@ P  ^ ` p@  ^` #B$B%B-B.BBMBNB\B]B^BfBBBBBBBBBCCC CCCCC'C(C6C7C8C?CCCCCضثؚ؉xgث j8h~CJU\aJ jh~CJU\aJ jLh~CJU\aJ jh~CJU\aJh~>*䴳a j`h~CJU\aJ jh~CJU\aJh~CJ\aJjh~CJU\aJ jth~CJU\aJ'CDDDDDDDWDDDDDDDDD E E E EEEEEEERGGؼ؈zn]إRh~6CJ\aJ jh~CJU\aJhoh~CJ\aJhJXh~>*䴳ahJXh~CJ\aJ jhO/CJU\aJhO/CJ\aJjhO/CJU\aJh~>*䴳a j$h~CJU\aJh~CJ\aJjh~CJU\aJ jh~CJU\aJGGXHHHHXIYIgIhIiIIIIIIIIIIIIIIIIDJUJVJdJeJfJJ᭾៖tcᖾR jfh~CJU\aJ jh~CJU\aJ jvh~CJU\aJ jhO/CJU\aJhO/CJ\aJjhO/CJU\aJ jh~CJU\aJjh~CJU\aJ h~CJ\h~B*CJ\aJphfh~CJ\aJh~6CJ\aJhO/6CJ\aJ WIXIJJJJKKKKKLEMMNQOOOPPPPPQkQmQ h^h` 0^`0JJJJJKKK)K*K+K[KKKKKKKK\LcLLLLMDM[MMMRNYNNNOOPOnOOOOOOP|P}PPPPPPPjhTCJU\aJ jh~CJU\aJ jVh~CJU\aJ jh~CJU\aJjh~CJU\aJhTCJ\aJh~>*䴳ah~CJ\aJh~5CJ\aJhO/CJ\aJ2PPPPQQQ RRURoRRSSSSSSSSSSSSTTT UURUkUUUUV_V}VVVV WjWqWWWX.X\XzXXXXYYYYYͻͪᡐ j6hTCJU\aJhTCJ\aJ jh~CJU\aJjh~CJU\aJh~h~CJ\aJh~>*䴳ajhTCJU\aJ jFhTCJU\aJ8mQQQVRRRKSMSaSbSSSTRUUVVVVW]XXZY[YYYYY$-DM a$gd~YYYYYYY`ZeZ\]]/]Z][]`]e]]]}___````bbbedgdyddeeeeNeRefffffVgjj:krksktkvkwk涱ߐh2 jh2 Uh~wh~B*\phhjh5CJhjh~5CJ h~5hjh~5 h5CJh~5>*CJ hT5CJh~56CJ] h~CJ\ h~5CJh~CJ\aJhTCJ\aJ4YYYY?ZrZtZZZ[\\\\f]]]]____E`F`` ^ gd~@^@ ^    ^ `  ` ```aWaaaabbbeefffffjjjjjjj:k  h^h` & F 8^8`:kskukvkxkyk{k|k~kkkkkkkkkkh]h&`#$ gd~wkykzk|k}kkkkkkkkkkkkkkkkh~wh~B*\phjh~0JU*hQA^0JmHnHu*h~ h~0Jjh~0JUjh2 Uh2 ,1h/ =!8"8#$`% $$If!vh#v$ #v\ #v:V lC t065$ 5\ 5a$$If!vh#v$ #v\ #v:V l t065$ 5\ 5a$$If!vh#v$ #v\ #v:V l t065$ 5\ 5a$$If!vh#v$ #v\ #v:V l t065$ 5\ 5a$$If!vh#v#v,:V l t0655,a$$If!vh#v#v,:V l t0655,a$$If!vh#v#v,:V l t0655,a$$If!vh#v#v,:V l t0655,a $$If!vh#v(#v#vp:V >  t20'5(55pp2ytJ$$If!vh#v(#v#vp:V > t0'5(55p$$If!vh#v(#v#vp:V > t0'5(55p$$If!vh#v(#v#vp:V > t0'5(55p$$If!vh#v(#v#vp:V l t0'5(55pvDeCheck13vDeCheck14vDeCheck15vDeCheck16vDeCheck17vDeCheck18vDeCheck19vDeCheck20vDeCheck21vDeCheck22vDeCheck23vDeCheck24vDeCheck25vDeCheck26vDeCheck27vDeCheck28vDeCheck29vDeCheck30vDeCheck31vDeCheck32vDeCheck33vDeCheck34vDeCheck35vDeCheck36vDeCheck37vDeCheck38vDeCheck39vDeCheck40vDeCheck41vDeCheck42vDeCheck43vDeCheck44xDeCheck115xDeCheck116xDeCheck117xDeCheck118xDeCheck122xDeCheck156xDeCheck157xDeCheck123xDeCheck124xDeCheck158xDeCheck159s666666666vvvvvvvvv666666>6666666666666666666666666666666666666666666666666hH6666666666666666666666666666666666666666666666666666666666666666666866666662 0@P`p2( 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p(8HX`~ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@_HmH nH sH tH @`@ NormalCJ_HaJmH sH tH ::  Heading 1$@&5\@@  Heading 2$$@&a$5\H@H  Heading 3$$@&a$5CJ\aJDA`D Default Paragraph FontVi@V 0 Table Normal :V 44 la (k ( 0No List 4B@4 Body Text5\DCD Body Text Indent @^@NRN Body Text Indent 2  ^ 5\FP@"F Body Text 2$a$5CJ\aJ>Q@2> Body Text 3 CJ\aJ4 @B4 Footer  !.)@Q. Page Number\S@b\ Body Text Indent 3h^h` CJ\aJForF HTML BodyOJQJ_HmH sH tH *W`* p`Strong5\88 5tauthorsdd[$\$4U 4 5t0 Hyperlink >*phPK![Content_Types].xmlN0EH-J@%ǎǢ|ș$زULTB l,3;rØJB+$G]7O٭VvnB`2ǃ,!"E3p#9GQd; H xuv 0F[,F᚜K sO'3w #vfSVbsؠyX p5veuw 1z@ l,i!b I jZ2|9L$Z15xl.(zm${d:\@'23œln$^-@^i?D&|#td!6lġB"&63yy@t!HjpU*yeXry3~{s:FXI O5Y[Y!}S˪.7bd|n]671. tn/w/+[t6}PsںsL. J;̊iN $AI)t2 Lmx:(}\-i*xQCJuWl'QyI@ھ m2DBAR4 w¢naQ`ԲɁ W=0#xBdT/.3-F>bYL%׭˓KK 6HhfPQ=h)GBms]_Ԡ'CZѨys v@c])h7Jهic?FS.NP$ e&\Ӏ+I "'%QÕ@c![paAV.9Hd<ӮHVX*%A{Yr Aբ pxSL9":3U5U NC(p%u@;[d`4)]t#9M4W=P5*f̰lk<_X-C wT%Ժ}B% Y,] A̠&oʰŨ; \lc`|,bUvPK! ѐ'theme/theme/_rels/themeManager.xml.relsM 0wooӺ&݈Э5 6?$Q ,.aic21h:qm@RN;d`o7gK(M&$R(.1r'JЊT8V"AȻHu}|$b{P8g/]QAsم(#L[PK-![Content_Types].xmlPK-!֧6 0_rels/.relsPK-!kytheme/theme/themeManager.xmlPK-!R%theme/theme/theme1.xmlPK-! ѐ' theme/theme/_rels/themeManager.xml.relsPK] b $$$' ~ TI&,l0 ;@#BCGJPYwkk68:;DKMOQRTUVXY[_ X0&1&8&?&F&T'. 9@WImQY`:kk79<=>?@ABCEFGHIJLNPSWZ\]^####`$p$$$n&~&'')/)++--22z667&7/7?7L7\7i7y7777777888888999)999I999999 ::#::::;;;; <D@T@r@@@@@@AAQABBBBhGxGGGrJJJJbG$G G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G$G G$G$G G$G$G$G$G$G$G G$G  '!!8@0(  B S  ?+Check13Check14Check15Check16Check17Check18Check19Check20Check21Check22Check23Check24Check25Check26Check27Check28Check29Check30Check31Check32Check33Check34Check35Check36Check37Check38Check39Check40Check41Check42Check43Check44Check115Check116Check117Check118Check122Check156Check157Check123Check124Check158Check159##a$$o&' )+-2{6707M7j777788899:9999:::;;E@s@@@BABBiGGsJJb  !"#$%&'()*##q$$&'0)+-26'7@7]7z77778889*9J999 :$::;; <U@@@@RABByGGJJb//E/M/0$0^7h7@@YY_babbbdbebgbhbjbkbbboz  "";%%%l&((+,1N2c22433O4Q45x6(7.7778899; ;<<@@xA{ANCCC/DFDDDEEF;FZIIK;LLLhMM\NNOGOOOPP#Q)Q+Q.QQQWQQRTT=\A\_babbbdbebgbhbjbkbbb333333333333333333333333333333333333333333`h%,MMOVWWeevz ##%$\$_$_$;;;;;;<<>>r@@0AAArAyAAABBFFGGYHYHHHJJKKpLpLPPPPLQQQFTGTITITLTQTVVW WxW}WWWbXbX[[:\>\@\@\aa^b_b_bbbbbkbsbvbbbw qJe0poDN,=lĀ'rR7AR*Μ=bu+mCr(va{~0"8;B>'<h FZ;&K@ {kaPdm ~8YpVihv jLdwglǢ,f7o."u$qv .lIw¡x3|V^`o(.rr^r`.B LB ^B `L.  ^ `.^`.L^`L.^`.RR^R`."L"^"`L.^`5o( ^`hH. pLp^p`LhH. @ @ ^@ `hH. ^`hH. L^`LhH. ^`hH. ^`hH. PLP^P`LhH.^`5o(. ^`hH. pLp^p`LhH. @ @ ^@ `hH. ^`hH. L^`LhH. ^`hH. ^`hH. PLP^P`LhH.^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.%%^%`o(.  ^ `. L ^ `L.^`.ee^e`.5L5^5`L.^`.^`.L^`L.^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L. ^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.^`o(.XX^X`.(L(^(`L.  ^ `.  ^ `.L^`L.hh^h`.88^8`.L^`L.@h^`56)^`5o(.XX^X`.(L(^(`L.  ^ `.  ^ `.L^`L.hh^h`.88^8`.L^`L. ^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L. @M^`M5.^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.  \ ^ `\o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.808^8`0o(. ^`hH. pLp^p`LhH. @ @ ^@ `hH. ^`hH. L^`LhH. ^`hH. ^`hH. PLP^P`LhH.  \ ^ `\o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.^`OJPJQJ^Jo( ^`OJQJo(o pp^p`OJQJo( @ @ ^@ `OJQJo( ^`OJQJo(o ^`OJQJo( ^`OJQJo( ^`OJQJo(o PP^P`OJQJo(^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.^`o(.^`.pLp^p`L.@ @ ^@ `.^`.L^`L.^`.^`.PLP^P`L.i&KCr(f7o7A j+'r$q ~8YlIwx3|~0=w qh FdwglkaP8;BoZ        :,[        &                          &                          Rt        0                          C                         "                                   7/MG XKI=\N^7/Mk2  5EmO/W>F>=M[SQA^r`R,oEjJ;~t!sDITFI,_bab@(b@ @UnknownG.Cx Times New Roman5Symbol3. .Cx Arial?= .Cx Courier New;WingdingsA$BCambria Math"1 hIgIgfS2S2!84-b-b2QH?'2 JACKSON STATE UNIVERSITYResearch & DevelopmentMichael A Early`                Oh+'0 , L X d p|JACKSON STATE UNIVERSITYResearch & Development 71FE9CE6Michael A Early2Microsoft Office Word@F#@w@p=@p=S ՜.+,D՜.+,\ hp  Ƶ2-b JACKSON STATE UNIVERSITY Title<@0_AdHocReviewCycleID_EmailSubject _AuthorEmail_AuthorEmailDisplayName_ReviewingToolsShownOnceY5Information Requestmichael.a.early@jsums.edu Mike Early  !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`bcdefghijklmnpqrstuvwxyz{|}~Root Entry Fc==Data a1Tableo]WordDocument.SummaryInformation(DocumentSummaryInformation8CompObjr  F Microsoft Word 97-2003 Document MSWordDocWord.Document.89q